US Patent 7531317 Fluorescence polarization assay to detect protease cleavage
Patent 7531317 was granted and assigned to Wisconsin Alumni Research Foundation on May, 2009 by the United States Patent and Trademark Office.
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The invention discloses a method of determining protease activity, in real time, using fluorescence polarization technology. In particular, the invention provides vectors and a method for their use, which expresses uncharacterized proteins conjugated to a fluorescence tag, which binds specifically to a fluorescent ligand. Cleavage of the recombinant protein results in a fragment of the expressed peptide and results in a change in fluorescence polarization of the fluorophore. The rate of change in fluorescence polarization can be measured in real time and is equivalent to the rate of protease cleavage.
US Patent 07531317 Fluorescence polarization assay to detect protease cleavage US Patent 7531317 Fluorescence polarization assay to detect protease cleavage
US Patent 07531317 Fluorescence polarization assay to detect protease cleavage
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