Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Josefine Persson0
Philip M. Lester0
Date of Patent
August 25, 2009
0Patent Application Number
116095290
Date Filed
December 12, 2006
0Patent Primary Examiner
Patent abstract
A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.
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