Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Jason A. Wilken0
Elliott Bedows0
Date of Patent
October 6, 2009
0Patent Application Number
108871060
Date Filed
July 8, 2004
0Patent Primary Examiner
Patent abstract
The present invention relates to a two-step method for isolating proteins from the cystine-knot superfamily based on dye ligand affinity chromatography and reversed-phase chromatography. Advantageously, the method can be performed in a relatively short period of time, involves inexpensive reagents, and requires little sample preparation before and during the purification process. Protein fusions between cystine-knot proteins and proteins of interest are further provided for the isolation of said protein of interest or complexes containing said protein of interest using the two-step method disclosed.
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