Is a
Patent attributes
Patent Jurisdiction
Patent Number
Date of Patent
October 20, 2009
Patent Application Number
10522405
Date Filed
July 28, 2003
Patent Primary Examiner
Patent abstract
The invention relates to a method of determining of accurately determining the copy number of a nucleotide sequence I in a sample using an amplification technique, such as PCR. In addition, a second nucleotide sequence II is also measured and calibration curves for each are made, from which the relative copy number CN can be determined. According to the present invention, accuracy is improved by performing multiple amplifications in a single well using real time PCR.
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