Patent attributes
One or more diaphragm valves are integrated as part of a cover of a microfluidic cassette cartridge. A rigid body portion includes one or more recessed valve areas and a plurality of fluid channels. A recessed valve area is disposed along either a bottom surface or a top surface of the rigid body portion. An inlet fluid channel and an outlet fluid channel are formed within the rigid body portion of each recessed valve area. A flexible membrane cover is sealed over the bottom surface of the body portion, and another flexible membrane cover is sealed over the top surface. A portion of the membrane cover that covers a recessed valve area functions as a valve membrane of the diaphragm valve. The flexible membrane functions as both a cover of the cartridge, thereby sealing and enclosing the appropriate channels, and a valve membrane of the diaphragm valve.