Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Rudolf H. Aebersold0
Date of Patent
February 2, 2010
0Patent Application Number
104552460
Date Filed
June 4, 2003
0Patent Primary Examiner
Patent abstract
The invention provides methods for identifying and quantifying polypeptides in a sample. The methods include the steps of labeling peptides in a polypeptide sample with an isotope tag; adding a plurality of peptide standards to the polypeptide sample, wherein the peptide standards are labeled with an isotopically distinct version of the isotope tag; resolving the labeled sample and standard peptides into a plurality of fractions; analyzing the resolved fractions using mass spectrometry; identifying an isotope-tagged sample peptide in an analyzed fraction; and determining the amount of the identified isotope-tagged sample peptide in the analyzed fraction by comparison to the amount of isotope tagged standard peptide in the same fraction.
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