A manually operated bicycle storage and retrieval assembly for loading, storing and unloading bikes. The storage assembly is mountable within or to the top of existing bicycle storage racks. The storage assembly has a frame, a lower track, an upper track and a dampening system which interconnects the lower and upper trolley track. The upper track has front and rear bike wheel securement structures which secure a bike to the upper track. A cooperating tool assembly is provided to operate the bicycle storage assembly and to pivot the upper track with respect to the lower track to load and unload bikes.