A radio frequency (RF) testing system (100) includes a RF signal source (10) generating RF signals, an antenna (50), a RF testing circuit (40) disposed on a printed circuit board (PCB) (41), a testing probe (20) for receiving the RF signal from the RF testing circuit, and a testing apparatus (30) for testing the RF signals from the testing probe. The RF testing circuit includes a first pad (42), a second pad (44), a transmission line (48), and at least one ground portion (49) parallel to the transmission line. A distance between a first region defined by the first pad and a second region defined by the second pad positioned between the first region and the antenna is equal to one-fourth of the wavelength. During testing, the testing probe is electrically connected to the first pad, and the second pad is electrically connected to ground of the PCB.