US Patent 7785884 Low density spreading methods for conifer somatic embryogenesis
Patent 7785884 was granted and assigned to Weyerhaeuser on August, 2010 by the United States Patent and Trademark Office.
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In one aspect, the present invention provides methods of producing conifer cotyledonary somatic embryos from pre-cotyledonary embryos. The methods of this aspect of the invention include the step of (a) dispensing a plurality of pre-cotyledonary embryos onto a porous material horizontally disposed over a non-porous surface in a volume of sterile dilution medium sufficient to submerge at least the surface of the porous material, thereby uniformly dispersing the pre-cotyledonary embryos; (b) removing the sterile dilution medium from the non-absorbent porous material, thereby trapping the uniformly dispersed pre-cotyledonary embryos on the porous material; and (c) contacting the pre-cotyledonary embryos trapped on the porous material with development medium for a period of time sufficient to produce conifer cotyledonary somatic embryos.
US Patent 07785884 Low density spreading methods for conifer somatic embryogenesis US Patent 7785884 Low density spreading methods for conifer somatic embryogenesis
US Patent 07785884 Low density spreading methods for conifer somatic embryogenesis
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