Patent 7824878 was granted and assigned to Charm Sciences, Inc. on November, 2010 by the United States Patent and Trademark Office.
Methods are provided for sensitive detection of adenosine triphosphate (ATP) in samples using the luciferin-luciferase reaction. Aspects include using a pH composition that maximizes a signal to noise ratio. The maximum signal to noise ratio can be particularly useful with recombinant luciferase including recombinant Coleoptera luciferase.