US Patent 7847087 Methods and primers for evaluating HIV-1 mutations
Patent 7847087 was granted and assigned to Siemens Healthcare Diagnostics Inc. on December, 2010 by the United States Patent and Trademark Office.
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Primer sequences and a method of using such sequences for the genotyping of HIV-1-containing samples, particularly those which have failed genotyping analysis are provided using primer sequences designed for analysis of Group B subtype of the Group M type virus. For example, a combination of primers, including at least one species of forward primer and at least one species of reverse primer where the forward primer(s) can be represented by the degenerate sequence: RARRARGGGCTGYTGGARATGTS (Seq. ID No. 9) and the reverse primer(s) can be represented by the degenerate sequence: BCHTYACYTTRATCCCSGVRTARATYTGACT (Seq. ID No.: 10) or BCHTYACYTTRATCCCSGVRTARATYTGAC (Seq. ID No. 12) are suitably employed. The selected primers, one or more from each group, can be used as reverse transcription, amplification and sequencing primers and are suitably packaged in a genotyping kit. Such a kit may include reagents in addition to the primers, such as an RNase inhibitor, a reverse transcriptase, a polymerase, and/or dNTP and ddNTP feedstocks.
US Patent 07847087 Methods and primers for evaluating HIV-1 mutations US Patent 7847087 Methods and primers for evaluating HIV-1 mutations
US Patent 07847087 Methods and primers for evaluating HIV-1 mutations
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