US Patent 7892831 Method for in vitro mass culture of

The present invention provides a method for producing a true-to-type clone of an Aloe bαrbαdensis mother plant by selecting an Aloe bαrbαdensis mother plant; isolating a meristematic explant from the plant; culturing the meristematic explant in initiation medium to generate shoots, where the media lacks hormones; culturing the shoots in proliferation and elongation medium to generate elongated shoots, where the proliferation and elongation media comprises benzyl adenine (BA) and indole butyric acid (IBA); culturing the elongated shoots in rooting medium to generate plantlets, where the media lacks hormones; and culturing the plantlets to product a true-to-type clone of the Aloe bαrbαdensis mother plant.