An automatic registration device applied to screen printing machines includes a base disposed under the printing table and is arranged with at least three sets of CCD (Charge Coupled Device), a printing table and an automatic registration member. The two sets of CCD are arranged on one side of the base while the other one set of CCD is disposed on the other side. The printing table is for loading and positioning the articles to be printed and is disposed with through holes corresponding to the image capture devices respectively as well as light sources for emitting light upward and the light passing through the insertion hole. The automatic registration member with a screen is arranged over the printing table. The CCD takes images of the article to be printed and the screen at the same time by the through hole. Through the computer, the automatic registration member and the screen move synchronously for accurate positioning to the article to be printed.