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US Patent 7947655 Methods for making proteins containing free cysteine residues

Patent 7947655 was granted and assigned to Bolder Biotechnology on May, 2011 by the United States Patent and Trademark Office.

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Patent abstract

The present invention relates to novel methods of making soluble proteins having free cysteines in which a host cell is exposed to a cysteine blocking agent. The soluble proteins produced by the methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form pegylated proteins.

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