Is a
Patent attributes
Patent Jurisdiction
Patent Number
Date of Patent
August 23, 2011
Patent Application Number
11990115
Date Filed
August 2, 2006
Patent Primary Examiner
Patent abstract
The present invention discloses a two-step enzyme method for preparing 7-aminocephalosporanic acid from cephalosporin C, wherein D-amino acid oxidase used is purified D-amino acid oxidase mutant of yeast Trigonopsis variabilis, having a specific activity of 105% higher than that of parent D-amino acid oxidase. The method has no need of addition of hydrogen peroxide, β-lactamase inhibitor, catalase inhibitor, catalase and the like commonly used in the prior art. The productivity of the method can reach more than 93%. Thus, the method is simple, low in cost and high in productivity.
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