Is a
Patent attributes
Current Assignee
Patent Jurisdiction
Patent Number
Patent Inventor Names
Niall A. Armes0
Colin H. Williams0
Derek L. Stemple0
Olaf Piepenburg0
Date of Patent
September 13, 2011
0Patent Application Number
128028620
Date Filed
June 14, 2010
0Patent Citations Received
Patent Primary Examiner
Patent abstract
This disclosure describes three related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundreds of megabases in length.
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