US Patent 8134705 Particle imaging systems and methods using acoustic radiation pressure

A method for imaging acoustically manipulated particles includes introducing a fluid containing a population of particles therein to a flow cell; applying acoustic radiation pressure to the flow cell; acoustically focusing the population of particles within the flow cell to concentrate the population of particles; interrogating some of the concentrated population of particles with an interrogation source to obtain an optical signal from some of the population of particles to yield population statistical data; and constructing a sampling matrix of particles from subpopulations of the concentrated population of particles to produce a high content image representative of the population of particles.