US Patent 8183359 Kits for amplifying DNA
Patent 8183359 was granted and assigned to Gen-Probe on May, 2012 by the United States Patent and Trademark Office.
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Kits for amplifying DNA which include a priming oligonucleotide that hybridizes to a 3′-end of a DNA target sequence, a displacer oligonucleotide that hybridizes to a target nucleic acid containing the DNA target sequence at a position upstream from the priming oligonucleotide, and a promoter oligonucleotide that includes a region that hybridizes to a 3′-region of a DNA primer extension product that includes the priming oligonucleotide and a promoter for an RNA polymerase. The priming oligonucleotide does not include an RNA region that hybridizes to the target nucleic acid and is selectively degraded by an enzyme activity when hybridized to the target nucleic acid. The kits do not include a restriction endonuclease and oligonucleotides that include a promoter for an RNA polymerase are all modified to prevent the initiation of DNA synthesis therefrom.
US Patent 08183359 Kits for amplifying DNA US Patent 8183359 Kits for amplifying DNA
US Patent 08183359 Kits for amplifying DNA
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