US Patent 8198093 Methods for sorting particles

A method of sorting stained particles in a flow cytometer by generating a mathematical model based upon extracted features of waveform pulses produced in response to the stained particles. Waveform pulses can then be analyzed to determine the probability the stained particles belong in either a first or a second population and a decision boundary can be established. Sorting decisions can be made based upon the extracted features of the waveform pulses relative to the decision boundary in combination with other desirability factors for particles or droplets containing the particles.