An imaging system includes: an image module configured to produce image frames at a frame rate F, the image module being configured to capture first and second portions of each of the image frames at different times; a frequency determination module, communicatively coupled to the image module, configured to determine frequency components associated with the first and second portions over a plurality, N, of the image frames; and a flicker analysis module, communicatively coupled to the frequency determination module, configured to determine whether any of the frequency components of the first and second portions are indicative of perceptible flicker.