An alignment device for a bag containing one or more ports wherein the alignment device is attached to the one or more ports or to the bag adjacent the one or more ports. The plate has either an indicator or a unique outer edge shape that is designed to fit into a corresponding unique opening in a bag holder to ensure proper alignment of the bag and its port(s) in the holder. In one embodiment the plate has a series of holes equal to and in alignment with the one or more ports of the bag and the port(s) are extended through the holes of the plate and the plate is attached to the bag by retainers on the port(s) below the plate. Alternatively, the plate may be fitted over the port(s) and attached to the bag.