Patent 9447445 was granted and assigned to NEW ENGLAND BIOLABS, INC. on September, 2016 by the United States Patent and Trademark Office.
This disclosure provides, among other things, a composition comprising: a 5′ exonuclease; a strand-displacing polymerase; and optionally a single strand DNA binding protein and/or a ligase. A method for polynucleotide assembly to form a synthon, as well as a kit for performing the same, are also described.