A universal golf cart windshield assembly includes a pair of elongated rails and a bracket secured to each rail, where each bracket is configured to clamp over a vertical support member of a golf cart. A longitudinal slot is disposed on each rail and a flange is joined along a longitudinal edge of the longitudinal slot and extends generally at a right angle to the slot. A plurality of apertures are disposed on the flange of each rail. The assembly also includes a lower windshield pane and an upper windshield pane, where opposing edges of the upper windshield pane are slidingly engaged with the respective longitudinal slot of each rail. In addition, the assembly also includes a spring loaded latch secured to the upper windshield pane, where the pin is adapted to engage a desired aperture of the flange to restrain the upper windshield pane in a desired vertical position.