Patent 9551023 was granted and assigned to Oxford Nanopore Technologies on January, 2017 by the United States Patent and Trademark Office.
The invention relates to a method for modifying a template polynucleotide for characterization, especially for nanopore sequencing. The method produces a modified polynucleotide which is complementary to the template polynucleotide at some positions and which contains universal or abasic nucleotides at the other, and in some instances predicable, positions. The resulting modified polynucleotide can then be characterized.