Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Mats Nilsson Bernitz0
Chatarina Larsson0
Ida Grundberg0
Date of Patent
January 31, 2017
Patent Application Number
13973814
Date Filed
August 22, 2013
Patent Citations Received
Patent Primary Examiner
Patent abstract
Embodiments relate to the detection of RNA in a sample of cells. More particularly, methods concern the localized detection of RNA in situ. The method relies on the conversion of RNA to complementary DNA prior to the targeting of the cDNA with a padlock probe(s). The hybridization of the padlock probe(s) relies on the nucleotide sequence of the cDNA which is derived from the corresponding nucleotide sequence of the target RNA. Rolling circle amplification of the subsequently circularized padlock probe produces a rolling circle product which may be detected. Advantageously, this allows the RNA to be detected in situ. In additional methods, rolling circle amplification products are sequenced.
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