Provided are a reel-to-reel inspection apparatus and method. The reel-to-reel inspection apparatus includes: an unwinding unit configured to unwind a roll-shaped object; a first inspection unit configured to photograph a surface of the object discharged from the unwinding unit; a second inspection unit configured to photograph another surface of the object which has passed through the first inspection unit; a marking unit configured to indicate a mark on the object which has passed through the second inspection unit; and a winding unit configured to wind in a roll shape the object which has passed through the marking unit.