A reversible makeup brush holder includes a base body for receiving water. The middle of the base body is connected with a support part. The insertion seat includes a disc body having an accommodation hole. Upper and lower surfaces of the disc body are provided with a rotary shaft, respectively. The rotary shaft is pivoted to the support part. Through the aforesaid design, a makeup brush can be inserted into the insertion hole. The insertion seat can be lifted and overturned, so that the water remaining in the bristles of the makeup brush can drip down rapidly on the base body. The user can clean the makeup brush holder conveniently.