Patent attributes
A method prepares a sugar solution from cellulose-containing biomass. The method includes step (1): a step of obtaining an endoxylanase hydrolysate by hydrolyzing the cellulose-containing biomass using endoxylanase derived from microorganisms of the Acremonium genus or the Aspergillus genus, step (2): a step of separating the endoxylanase hydrolysate into an endoxylanase hydrolysate solid and an endoxylanase hydrolysate liquid through solid-liquid separation, step (3): a step of obtaining a cellulase hydrolysate by hydrolyzing the endoxylanase hydrolysate solid using cellulase derived from a filamentous fungus, and step (4): a step of filtering the cellulase hydrolysate through an ultrafiltration membrane to recover a sugar solution from the filtrate side and collect an enzyme component from the non-filtrate side.