An eyeglasses assembly structure having a nose support is disclosed herein. It comprises a frame having an assembly holder disposed in a middle bottom portion thereof, wherein the assembly holder is provided with a receiving slot, and the receiving slot is provided with a first inner surface having a protrusion thereof and a second inner surface having a recess thereon; and a nose support having an inserting part for inserting into the receiving slot of the assembly holder of the frame, wherein the inserting part is provided with a first outer surface having an engaging slot for correspondingly engaging with the protrusion and a second outer surface having an engaging block for correspondingly engaging with the recess.