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Eleanor of Toledo was born on January 11, 1522, in Toledo, Spain . She was a Spanish noblewoman and the first wife of Cosimo I de' Medici . Eleanor was the daughter of Pedro Álvarez de Toledo, Marquis of Villafranca .
During her husband's frequent absences, Eleanor ruled as regent of Florence . She held Spanish and Florentine nationalities. Throughout her life, Eleanor had several children, including Ferdinando I de' Medici, Grand Duke of Tuscany , Francesco I de' Medici , and Isabella de' Medici .
Along with her other children, she was the mother to Maria de' Medici , Giovanni de' Medici , Lucrezia de' Medici , Antonio de' Medici , Garzia de' Medici , and Pietro de' Medici .
Eleanor of Toledo passed away on December 17, 1562, in Pisa .