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US Patent 11661623 Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use

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Patent Applicant
Promega
Promega
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Current Assignee
Promega
Promega
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Patent Jurisdiction
United States Patent and Trademark Office
United States Patent and Trademark Office
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Patent Number
116616230
Date of Patent
May 30, 2023
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Patent Application Number
170115970
Date Filed
September 3, 2020
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Patent Citations
‌
US Patent 7429472 Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule
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US Patent 7537912 Methods of measuring luminogenic activity with a protected coelenterazine
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US Patent 7553632 Luminogenic and nonluminogenic multiplex assay
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US Patent 7692002 Metal complexes having vitamin B
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US Patent 7692022 Luminescence-based methods and probes for measuring cytochrome P450 activity
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US Patent 7728118 Synthetic nucleic acid molecule compositions and methods of preparation
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US Patent 7867726 Compositions comprising a dehalogenase substrate and a fluorescent label and methods of use
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US Patent 7879540 Synthetic nucleic acid molecule compositions and methods of preparation
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...
Patent Primary Examiner
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David Steadman
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An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type

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