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US Patent 7537912 Methods of measuring luminogenic activity with a protected coelenterazine

Patent 7537912 was granted and assigned to Promega (company) on May, 2009 by the United States Patent and Trademark Office.

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Patent abstract

A method of measuring the enzymatic activity of a luciferase includes contacting a luminogenic protein, such as a luciferase, with a protected luminophore to form a composition; and detecting light produced from the composition. The protected luminophore provides increased stability and improved signal-to-background ratios relative to the corresponding unmodified coelenterazine.

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