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US Patent 12006520 Evaluation and improvement of nuclease cleavage specificity

Patent 12006520 was granted and assigned to President and Fellows of Harvard College on June, 2024 by the United States Patent and Trademark Office.

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Patent abstract

Engineered nucleases are promising tools for genome manipulation and determining off-target cleavage sites of these enzymes is of great interest. This disclosure provides in vitro selection methods that interrogate 10

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