US Patent 7138562 Transgenic animals

Patent 7138562 was granted and assigned to Wisconsin Alumni Research Foundation on November, 2006 by the United States Patent and Trademark Office.

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Anthony W. S. Chan0

Jane C. Burns0

Robert D. Bremel0

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Patent abstract

The present invention provides improved methods and compositions for the generation of transgenic non-human animals. The present invention permits the introduction of exogenous nucleic acid sequences into the genome of unfertilized eggs (e.g., pre-maturation oocytes and pre-fertilization oocytes) by microinjection of infectious retrovirus into the perivitelline space of the egg. The methods of the present invention provide an increased efficiency of production of transgenic animals with a reduced rate of generating animals which are mosaic for the presence of the transgene.

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https://pdfpiw.uspto.gov/.piw?Docid=071385620

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