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US Patent 7476500 In vivo selection system for enzyme activity

Patent 7476500 was granted and assigned to President and Fellows of Harvard College on January, 2009 by the United States Patent and Trademark Office.

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Patent abstract

The present invention provides in vivo systems in which activity of a biological cleavage enzyme, such as a site-specific recombinase, a homing endonuclease, or an intein, is linked to cell viability and therefore can be selected. The invention further provides methods of making cells in which the activity of a biological cleavage enzyme is linked to viability, as well as methods of identifying new biological cleavage enzymes, including enzymes having altered site specificity, using such cells.

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