Patent 7491409 was granted and assigned to TRANSAVE, INC. on February, 2009 by the United States Patent and Trademark Office.
This invention provides a method to prepare liposome-encapsulated bioactive agents such as nucleic acids, comprising complexation of the bioactive agents in reverse micelles prior to forming liposomes, as well as methods of using the liposomes so formed and formulations to deliver nucleic acids to cells.