US Patent 7498136 Methods for detecting multiple species and subspecies of

Disclosed are methods, kits, and other components for detecting multiple nucleic acids in a sample. The methods and kits may be useful for detecting Neisseria in a sample. In some embodiments, the methods include (a) reacting a mixture that includes (i) nucleic acid isolated from the sample; (ii) at least a first specific primer capable of being used to amplify specifically nucleic acid of Neisseria gonorrhea; (iii) at least a second specific primer capable of being used to amplify specifically nucleic acid of a non-gonococcal species of Neisseria; (iv) at least a universal primer capable of being used to amplify specifically nucleic acid of Neisseria gonorrhea and nucleic acid of a non-gonococcal species of Neisseria; and (b) amplifying and detecting nucleic acid of at least one of Neisseria gonorrhea and a non-gonococcal species of Neisseria. The disclosed kits may include one or more components for performing the disclosed methods.