Patent 7498136 was granted and assigned to ERAGEN BIOSCIENCES, INC. on March, 2009 by the United States Patent and Trademark Office.
Disclosed are methods, kits, and other components for detecting multiple nucleic acids in a sample. The methods and kits may be useful for detecting Neisseria in a sample. In some embodiments, the methods include (a) reacting a mixture that includes (i) nucleic acid isolated from the sample; (ii) at least a first specific primer capable of being used to amplify specifically nucleic acid of Neisseria gonorrhea; (iii) at least a second specific primer capable of being used to amplify specifically nucleic acid of a non-gonococcal species of Neisseria; (iv) at least a universal primer capable of being used to amplify specifically nucleic acid of Neisseria gonorrhea and nucleic acid of a non-gonococcal species of Neisseria; and (b) amplifying and detecting nucleic acid of at least one of Neisseria gonorrhea and a non-gonococcal species of Neisseria. The disclosed kits may include one or more components for performing the disclosed methods.