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US Patent 7745125 2′-terminator related pyrophosphorolysis activated polymerization

Patent 7745125 was granted and assigned to Roche Molecular Systems on June, 2010 by the United States Patent and Trademark Office.

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Patent abstract

The present invention provides reaction mixtures that include blocked oligonucleotides comprising 2′-terminator nucleotides. The blocked oligonucleotides are rendered extendible when the 2′-terminator nucleotides are removed from the oligonucleotides, e.g., via pyrophosphorolysis. The reaction mixtures can be used in various nucleic acid polymerization and/or amplification assays, among many other applications. In addition to reaction mixtures, the invention also provides related methods and reaction mixtures.

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