Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Janice M. Cline0
Holly H. Hogrefe0
Date of Patent
November 29, 2011
Patent Application Number
10198449
Date Filed
July 18, 2002
Patent Citations Received
0
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Patent Primary Examiner
Patent abstract
The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
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