Patent 8163529 was granted and assigned to NEW ENGLAND BIOLABS, INC. on April, 2012 by the United States Patent and Trademark Office.
A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or glutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6.