Is a
Patent attributes
Patent Jurisdiction
Patent Number
Date of Patent
October 20, 2015
Patent Application Number
12672606
Date Filed
August 8, 2008
Patent Citations Received
Patent Primary Examiner
Patent abstract
The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
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