Is a
Patent attributes
Patent Jurisdiction
Patent Number
Patent Inventor Names
Atsushi Sogabe0
Yoshihisa Kawamura0
Yoshiaki Nishiya0
Takashi Hattori0
Date of Patent
October 17, 2006
0Patent Application Number
108072280
Date Filed
March 23, 2004
0Patent Primary Examiner
Patent abstract
A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H2O→sarcosine+ureaOptimum temperature: about 40-50° C.Optimum pH: pH about 8.0-9.0Heat stability: not more than 50° C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.00 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: 3.5 4.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.
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