US Patent RE39352 Creatine amidinohydrolase, production thereof and use thereof

A creatine amidinohydrolase having the following physicochemical properties:Action: catalyzing the following reaction;creatine+H2O→sarcosine+ureaOptimum temperature: about 40-50° C.Optimum pH: pH about 8.0-9.0Heat stability: not more than 50° C. (pH 7.5, 30 min)Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5-10.00 mMMolecular weight: about 43,000 (SDS-PAGE)Isoelectric point: 3.5 4.5,a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor.